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icell8 cx single cell system  (TaKaRa)


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    Structured Review

    TaKaRa icell8 cx single cell system
    Icell8 Cx Single Cell System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/icell8+cx+system/ICELL8+cx+TCR+Chip/pmc12782168-51-24-29
    Average 94 stars, based on 15 article reviews
    icell8 cx single cell system - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Normal breast tissues harbour rare populations of aneuploid epithelial cells.
    Article Snippet: Aneuploid epithelial cells are common in breast cancer; however, their presence in normal breast tissues is not well understood.. To address this question, we applied single-cell DNA sequencing to profile copy number alterations in 83,206 epithelial cells from the breast tissues of 49 healthy women, and we applied single-cell DNA and assay for transposase-accessible chromatin sequencing co-assays to the samples of 19 women.. Our data show that all women harboured rare aneuploid epithelial cells (median 3.19%) that increased with age.

    Article Title: Normal Breast Tissues Harbor Rare Populations of Aneuploid Epithelial Cells
    Article Snippet: .. The isolated nuclei were resuspended in 0.5× Dulbecco’s PBS to a concentration of 40 cells ul −1 , then micro-dispensed into a nanowell chip using the ICELL8 cx system (Takara Bio), followed by centrifugation at 1,000 g for 5 min at 4 °C. .. The chip was scanned and imaged under DAPI/blue channel, then wells containing single nuclei were identified using CellSelect Software (Takara Bio) for subsequent reagent dispensing steps.

    Concentration Assay:

    Article Title: Normal breast tissues harbour rare populations of aneuploid epithelial cells.
    Article Snippet: Aneuploid epithelial cells are common in breast cancer; however, their presence in normal breast tissues is not well understood.. To address this question, we applied single-cell DNA sequencing to profile copy number alterations in 83,206 epithelial cells from the breast tissues of 49 healthy women, and we applied single-cell DNA and assay for transposase-accessible chromatin sequencing co-assays to the samples of 19 women.. Our data show that all women harboured rare aneuploid epithelial cells (median 3.19%) that increased with age.

    Article Title: Normal Breast Tissues Harbor Rare Populations of Aneuploid Epithelial Cells
    Article Snippet: .. After tagmentation, the nuclei were washed and diluted to a concentration of 40 cells per microlitre, then micro-dispensed into a nanowell chip using the ICELL8 cx system (Takara Bio), followed by five dispensing steps as described in the above DNA&ATAC-seq co-assay study, including lysis, tagmentation, neutralization, index dispensing and library PCR. ..

    Article Title: Normal Breast Tissues Harbor Rare Populations of Aneuploid Epithelial Cells
    Article Snippet: .. The isolated nuclei were resuspended in 0.5× Dulbecco’s PBS to a concentration of 40 cells ul −1 , then micro-dispensed into a nanowell chip using the ICELL8 cx system (Takara Bio), followed by centrifugation at 1,000 g for 5 min at 4 °C. .. The chip was scanned and imaged under DAPI/blue channel, then wells containing single nuclei were identified using CellSelect Software (Takara Bio) for subsequent reagent dispensing steps.

    Centrifugation:

    Article Title: Normal breast tissues harbour rare populations of aneuploid epithelial cells.
    Article Snippet: Aneuploid epithelial cells are common in breast cancer; however, their presence in normal breast tissues is not well understood.. To address this question, we applied single-cell DNA sequencing to profile copy number alterations in 83,206 epithelial cells from the breast tissues of 49 healthy women, and we applied single-cell DNA and assay for transposase-accessible chromatin sequencing co-assays to the samples of 19 women.. Our data show that all women harboured rare aneuploid epithelial cells (median 3.19%) that increased with age.

    Article Title: Archival single-cell genomics reveals persistent subclones during DCIS progression.
    Article Snippet: Resource Archival single-cell genomics reveals persistent subclones during DCIS progression

    Article Title: Normal Breast Tissues Harbor Rare Populations of Aneuploid Epithelial Cells
    Article Snippet: .. The isolated nuclei were resuspended in 0.5× Dulbecco’s PBS to a concentration of 40 cells ul −1 , then micro-dispensed into a nanowell chip using the ICELL8 cx system (Takara Bio), followed by centrifugation at 1,000 g for 5 min at 4 °C. .. The chip was scanned and imaged under DAPI/blue channel, then wells containing single nuclei were identified using CellSelect Software (Takara Bio) for subsequent reagent dispensing steps.

    Software:

    Article Title: Archival single-cell genomics reveals persistent subclones during DCIS progression.
    Article Snippet: Resource Archival single-cell genomics reveals persistent subclones during DCIS progression

    Lysis:

    Article Title: Normal breast tissues harbour rare populations of aneuploid epithelial cells.
    Article Snippet: Aneuploid epithelial cells are common in breast cancer; however, their presence in normal breast tissues is not well understood.. To address this question, we applied single-cell DNA sequencing to profile copy number alterations in 83,206 epithelial cells from the breast tissues of 49 healthy women, and we applied single-cell DNA and assay for transposase-accessible chromatin sequencing co-assays to the samples of 19 women.. Our data show that all women harboured rare aneuploid epithelial cells (median 3.19%) that increased with age.

    Article Title: Normal Breast Tissues Harbor Rare Populations of Aneuploid Epithelial Cells
    Article Snippet: .. After tagmentation, the nuclei were washed and diluted to a concentration of 40 cells per microlitre, then micro-dispensed into a nanowell chip using the ICELL8 cx system (Takara Bio), followed by five dispensing steps as described in the above DNA&ATAC-seq co-assay study, including lysis, tagmentation, neutralization, index dispensing and library PCR. ..

    Neutralization:

    Article Title: Normal breast tissues harbour rare populations of aneuploid epithelial cells.
    Article Snippet: Aneuploid epithelial cells are common in breast cancer; however, their presence in normal breast tissues is not well understood.. To address this question, we applied single-cell DNA sequencing to profile copy number alterations in 83,206 epithelial cells from the breast tissues of 49 healthy women, and we applied single-cell DNA and assay for transposase-accessible chromatin sequencing co-assays to the samples of 19 women.. Our data show that all women harboured rare aneuploid epithelial cells (median 3.19%) that increased with age.

    Article Title: Normal Breast Tissues Harbor Rare Populations of Aneuploid Epithelial Cells
    Article Snippet: .. After tagmentation, the nuclei were washed and diluted to a concentration of 40 cells per microlitre, then micro-dispensed into a nanowell chip using the ICELL8 cx system (Takara Bio), followed by five dispensing steps as described in the above DNA&ATAC-seq co-assay study, including lysis, tagmentation, neutralization, index dispensing and library PCR. ..

    Single Cell:

    Article Title: Archival single-cell genomics reveals persistent subclones during DCIS progression.
    Article Snippet: Resource Archival single-cell genomics reveals persistent subclones during DCIS progression

    DNA Library Preparation:

    Article Title: Archival single-cell genomics reveals persistent subclones during DCIS progression.
    Article Snippet: Resource Archival single-cell genomics reveals persistent subclones during DCIS progression

    Imaging:

    Article Title: Archival single-cell genomics reveals persistent subclones during DCIS progression.
    Article Snippet: Resource Archival single-cell genomics reveals persistent subclones during DCIS progression

    Polymerase Chain Reaction:

    Article Title: Normal Breast Tissues Harbor Rare Populations of Aneuploid Epithelial Cells
    Article Snippet: .. After tagmentation, the nuclei were washed and diluted to a concentration of 40 cells per microlitre, then micro-dispensed into a nanowell chip using the ICELL8 cx system (Takara Bio), followed by five dispensing steps as described in the above DNA&ATAC-seq co-assay study, including lysis, tagmentation, neutralization, index dispensing and library PCR. ..



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